Palladium induces oxidative stress and cell death in normal hepatocytes

چکیده مقاله

Environmental pollution of palladium (Pd) is increasing because of anthropogenic activities. It accumulate in many organs including liver, kidney and brain and renders many delirious effects. Although the mechanism of Pd toxicity has been investigated but the precise mechanism still need to be elucidated. Our findings cleared that the mechanism off Pd toxicity is due to inducing stress oxidative in mitochondrial and cell membrane and lysosomes. Findings of the current study elucidate that Pd toxicity on liver cell lysate is induced by ROS production, increasing lipid peroxidation and mitochondrial membrane potential reduction. These effects reversed via ROS scavengers DMSO and mannitol, mitochondrial permeability transmission (MPT) pore sealing agents including carnitine and trifluoperazine. Furthermore, L-glutamate, (a progenitor of ATP) inhibits toxic effects of Pd. Furthermore, the results indicated that Pd toxicity is mediated via mitochondrial pathway. Pretreatment of lysate via ROS scavengers reduced cell death and GSH depletion enhanced cell death that elucidate the rule of stress oxidative in Pd toxicity. Altogether, the results cleared the mechanism by which Pd-induced toxicity mechanism in the isolated rat hepatocytes.

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