- تاریخ انتشار : ۱۳۹۵
- ناشر : کنفرانس بین المللی پژوهش های نوین در علوم کشاورزی و محیط زیست
- زبان مقاله : همه
- تعداد صفحات : 9
- حجم فایل : 0 کیلوبایت
- نوع مقاله : مجموعه مقالات کنفرانس
- مجموعه : کشاورزی
چکیده مقاله
Cereals contamination of feedstuff such as corn with mycotoxigenic species of Fusarium is considered as a main source of fumonisin mycotoxin groups which cause worldwide serious diseases in human and animal health. According, in this study, a total of 100 Fusarium isolates were recovered from corn samples collected from 16 dairy farms in Razavy Khorasan Province, Iran, during 2011-2012. Fusarium isolates were identified on morphological and using species-specific PCR. Considering that, early detection of Fusarium species could be very important to prevent mycotoxin contaminating food and feed grains, a Multiplex-PCR assay was designed on the base of four sets of specific primers including: ITS-F/R, Vert1/2, FUM1-F/R and FP3-F/FP4-R which has been developed for the discovery of the two common Fusarium species (F.verticillioides and F.proliferatum) that they can potentially produce fumonisin. In this study, PCR analysis comfirmed 78% of morphological identification to species level as F.proliferatum (53.8%) and 29.5% as F.verticillioides. 42 (53.84%) of total (78) F.verticillioides and F.proliferatum isolated were fumonisin producing out of which 24 (57.14%) were F.proliferatum while 18 (42.86 %) were F.verticillioides. Moreover, we have established the method to discriminate between the two populations F.verticillioides and F.proliferatum, simultaneously enable us to discriminate fumonisin-producing strains from non-fumonisin-producing strains in the corn grains of animal feedstuff
نحوه استناد به مقاله
در صورتی که می خواهید به این مقاله در اثر پژوهشی خود ارجاع دهید، می توانید از متن زیر در بخش منابع و مراجع بهره بگیرید :
Asiyeh Mehrzad؛Seyed Ali Mortazavi؛Masumeh Mehraban Sangatash؛Roya Rezaeian Doloei؛Mohammad Mohsenzadeh؛ ۱۳۹۴، Multiplex-PCR detection of fumonisin-producing F.verticillioides and F.proliferatum in corn of feedstuff، کنفرانس بین المللی پژوهش های نوین در علوم کشاورزی و محیط زیست، https://scholar.conference.ac:443/index.php/download/file/561-Multiplex-PCR-detection-of-fumonisin-producing-F.verticillioides-and-F.proliferatum-in-corn-of-feedstuff
در داخل متن نیز هر جا به عبارت و یا دستاوردی از این مقاله اشاره شود پس از ذکر مطلب، در داخل پرانتز، مشخصات زیر نوشته شود.
(Asiyeh Mehrzad؛Seyed Ali Mortazavi؛Masumeh Mehraban Sangatash؛Roya Rezaeian Doloei؛Mohammad Mohsenzadeh؛ ۱۳۹۴)