Preparation and biological behavior of Chitosan:Starch biocomposite matrix modified by GLYMO for hard tissue engineering applications

چکیده مقاله

The goal of tissue engineering scaffolds is promotion of healing process of damaged tissues or organs. In this research, porous Chitosan:Starch scaffolds with weight ratio 50:50 prepared by freeze drying technique in which modified by organosilanes agents (GLYMO) with molar ratio polymer to GLYMO 1:0.5 to simulate extra cellular matrix (ECM). After that morphological and biological characters such as cellular adhesion, proliferation and ALP activity investigated. SEM micrographs of cellular scaffolds represented interconnected porous microstructure that is important parameter in cellular penetration, absorption of nutritious and excretion of waste products. Polymeric scaffolds showed approximately 95% porosity with average pore diameter of 120±36 μm. SEM micrographs of bone marrow mesanchymal stem cells indicated that polymeric scaffolds are biocompatible and support cellular adhesion, spreading and proliferation. Moreover, expression of alkaline phosphatase in modified constructs illustrated that biocomposite matrixes induce osteogenic differentiation. In brief, chitosan:starch:GLYMO scaffolds have biological features to regenerate bone defects.

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