Preliminary Phytochemical Screening, GC-MS analysis, Determination of Total phenolics, flavonoid content and Free Radical Scavenging Activities of Various Crude Extracts of Red Alga Hypnea boergesenii

چکیده مقاله

This study determined the total phenols and flavonoids, antioxidant activity, Preliminary Phytochemical Screening, GC-MS analysis. H. boergesenii red seaweed, collected from Chabahar Sea, on the coast of the Gulf of Oman. powder was extracted using five solvent: (15 g) with70 % ethanol and (340 g) extracted successively with four solvent (Hexane, petroleum ether, dichloromethane and methanol) using maceration method. all the crude extracts were concentrated under reduced pressure and dried by using freeze dryer. The antioxidant activity of the methanolic extract was measured using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and the IC50 is (17.68± 0.33). The total phenolics and total flavonoids content were also determined by Folin- Ciocalteu reagent and aluminum chloride colorimetric methods (TPC =3.42±0.04) and (TFC = 4.79 ± 0.11).
Phytochemical analysis of alcoholic extract showed presence of major classes of phytochemicals, such as: tannins, flavonoids, cardiac glycosides, steroids, etc. Gas chromatography-mass spectrometry results revealed presence phytoconstituents compounds in various extract of H. boergesenii alga.

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