Comparative study based on micro-dilution and plate count method for synergism antibacterial effect of MOF-5 nanoparticles and Broccoli extract Against Pseudomonas aeruginosa

چکیده مقاله

The aim of this work was comparison study of dilution and plating method for evaluation of the synergism effect of metal-organic framework nanoparticles (MOF-5 NPs) and broccoli extract (Brassica oleracea) on antibacterial activity of standard and clinical Pseudomonas aeruginosa strains. For this purpose, synthesis and characterization of MOF-5 NPs were performed using instrumental techniques including X-ray diffraction (XRD), FT-IR analysis and scanning electron microscopy (SEM). Maceration extraction (ME) and ultrasound assisted extraction (UAE) methods were used and compared for the preparation and evaluation of total phenolic content, reducing power, radical scavenging and antioxidant activity in three different solvents. The HPLC/UV analysis was applied for separation, identification and evaluation of phenolic acids in broccoli extract in two methods of UAE and ME. Then, antimicrobial activity of MOF-5 NPs and broccoli extract against gram-negative bacteria, Pseudomonas aeruginosa was evaluated by detection of minimal inhibition concentration (MIC), minimal bactericidal concentration (MBC) and zone of inhibition (ZOI). The results of in vitro assays showed that dilution method could not be able to estimate MBC. The reason for flase estimation of 4% viability percentage which is not logic by consideration of MBC well.

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